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Image Search Results
Journal: Advanced Science
Article Title: Molecular Design of a Naturally Derived Hemostatic Sealant with Prolonged Antimicrobial Activity for Repairing Elastic Organ Injuries
doi: 10.1002/advs.202506466
Figure Lengend Snippet: In vitro antibacterial properties and biocompatibility of GDP sealant. In vitro antimicrobial tests: A) Bacterial concentration measured by optical density (OD) at 625 nm of P. aeruginosa during 5 days of culture with either GDP hydrogels prepared with various concentrations of DMA and pDDA, broad‐spectrum antibiotic ciprofloxacin, or commercial wound dressing AquaDerm. B) Survival rate of P. aeruginosa after 5 days of treatment. C) Representative SEM images of GelMAG and GDP surfaces after 5 days of bacterial inoculation. D) Zone of inhibition (ZOI) formed by GelMAG or GDP against P. aeruginosa . E) OD measurements of MRSA during 5 days of culture with either GDP hydrogels prepared with various concentrations of DMA and pDDA, broad‐spectrum antibiotic ciprofloxacin, or commercial wound dressing AquaDerm. F) Survival rate of MRSA after 5 days of treatment. G) Representative SEM images of GelMAG and GDP surfaces after 5 days of bacterial inoculation. H) ZOI formed by GelMAG or GDP against MRSA. Negative control represented bacteria without hydrogel treatments. In vitro biocompatibility assessment: I) Representative live/dead stained images from NIH3T3 cells exposed to GelMAG or GDP through Transwell ® inserts for 5 days. Control cells were cultured without hydrogel exposure. J) Cellular viability after 1 and 5 days of incubation with either GelMAG or GDP hydrogels. K) Representative actin/DAPI stained images of NIH3T3 cells cultured with GelMAG or GDP for 5 days. L) Relative fluorescence unit (RFU) of cells cultured with GelMAG or GDP for 1, 3, and 5 days, assessed through PrestoBlue assay. Data are represented as mean ± SD. Analysis by two‐way ANOVA with Tukey's post‐hoc multiple comparisons test. * p < 0.05, ** p < 0.01, **** p < 0.0001. n = 3 biological replicates per group.
Article Snippet: In vitro biocompatibility studies were conducted using
Techniques: In Vitro, Concentration Assay, Inhibition, Negative Control, Bacteria, Staining, Control, Cell Culture, Incubation, Fluorescence, Prestoblue Assay